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KMID : 0352519920290020413
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Korea Univercity Medical Journal
1992 Volume.29 No. 2 p.413 ~ p.422
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Characteristics and Optimal condition in Primary Culture of Mouse Epithelial Cells
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Abstract
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This study was attempted to compare the characteristics of primary culture of mouse epithelial cells and to determine the effective seeding density for confluency on two substrates.
The dispersed single epithelial cells were cultured on culture dish coated with type III collagen and culture slide. The culture conditions were adjusted to 3 DMEM : 1 F12 media with 10% FBS, 37℃ temperature, 5% CO2 air and pH 7.2-7.4 but the
seeding
density was changed within 1×104-7×105 cells/cm2 substrate.
@ES The results were as follows:
@EN 1. The characteristics of cultured cells were very similar in the collagen coated dish-used group and the culture slide-used group. The proliferation, stratification, keratinization and desquamation processes were observed like those in vivo.
2. There was a slight tendency to promote attachment, proliferation and differentiation on the collagen coated dish as compared with the culture slide. But it was not statistically significant.
3. The effective seeding density was in the ragne of 4-5×105cell/cm2 substrate in both groups. But the time for confluency was shortened accordings to rasing the seeding density.
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KEYWORD
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